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![]() To gain a comprehensive understanding of regulation of immune response and resistance to NDV in chickens, however, we must also investigate gene expression response to NDV in other immune organs. Previously, we have compared the transcriptomic response to NDV in trachea between NDV-challenged and non-challenged chickens, as well as between the two chicken lines at 2, 6 and 10 dpi 13. Therefore, we hypothesize that Fayoumis are relatively resistant to NDV compared to Leghorns. When the two chicken lines were infected with the same amount of a B1 type La Sota Lentogenic NDV strain, Fayoumis showed faster viral clearing than Leghorns from 2 to 6 days post infection (dpi) and produced more antibody in serum at 10 dpi compared to Leghorns 13. Its robust resistance to some avian diseases, such as Salmonella 8, Eimeria 9, 10, Marek’s disease 11 and avian influenza 12 has been reported. The Fayoumi line was derived from Egyptian fowl imported to the U.S. Two genetically distant inbred chicken lines – Fayoumi and Leghorn – provide a discovery platform to investigate genetic regulation of response and resistance to NDV. ![]() The ability of the host to expel pathogens and to limit their proliferation and shedding are components of resistance 7. However, few studies have reported gene expression responses to NDV in different chicken lines. There have been studies investigating host genetic regulation in response to different NDV strains 5, 6. ![]() The severity of infection with the same NDV strain also varies between avian species and chicken breeds due to genetic variation 3. NDV strains can be classified from least to most virulent into lentogenic, mesogenic, and velogenic strains 4. The outcome of NDV infection depends on both the virulence of the NDV strain and the ability of chickens to resist to the virus. To ameliorate the negative impact of NDV, alleviate poverty and improve food security in these countries, genetic selection and breeding approaches can be utilized, in addition to NDV vaccination, to enhance resistance of chickens to NDV. In addition, advanced vaccines and reliable infrastructure for transportation and storage of the vaccine are usually scarce in developing countries 3, leading to mortality of chickens as high as 80% caused by the velogenic NDV that is endemic in these areas. Although vaccination has greatly reduced outbreaks of the endemic disease, it cannot prevent the continuous evolution of ND virus (NDV) 1 and some virulent strains can still survive in vaccinated chickens 2. Newcastle disease (ND) is a highly infectious disease that causes substantial economic losses to the global poultry industry. The identified DEGs and predicted pathways may contribute to differential responses to NDV between the two chicken lines and provide potential targets for breeding chickens that are more resistant to NDV. Pathways predicted by IPA analysis, such as "EIF-signaling", "actin cytoskeleton organization nitric oxide production" and "coagulation system" may contribute to resistance to NDV in Fayoumi chickens. Several interferon-stimulated genes were identified as important DEGs regulating immune response to NDV in chicken. At a false discovery rate <0.05, Fayoumi chickens, which are relatively more resistant to NDV, showed fewer differentially expressed genes (DEGs) than Leghorn chickens. Through transcriptome analysis, gene expression in spleen at 2 and 6 days post-inoculation was compared between NDV-infected and control groups, as well as between chicken lines. In this study, two inbred chicken lines with different responses to NDV, Fayoumi and Leghorn, were challenged with LaSota NDV strain at 21 days of age. Enhancing genetic resistance of chickens to Newcastle Disease Virus (NDV) provides a promising way to improve poultry health, and to alleviate poverty and food insecurity in developing countries. ![]() Sita was Bhumi Devi’s real daughter, Janak and Sunaina’s oldest daughter Ram’s wife and Urmila’s sister. The role of Sita in the Ramayan serial cast is played by Deepika Chikhalia. Sita was ideally considered to be Goddess Lakshmi’s incarnation. He subsequently returned for another screen test, this time with a smile on his face, and was cast in the role. Initially, he was thought to be unsuitable for the part. Govil expressed an interest in playing Rama and attended a screen test. The backstory of Govik being a part of the Ramayan cast is quite charming. Rama or Ram was Dasharatha and Kaushlya’s son, Sita’s husband and Lakshman, Bharat, and Shatrughan’s older brother. Let’s take a look at some of the most amazing actors who made the Ramayan serial a success as it is. The cast and their respective roles in the Ramayan serial have been discussed below: THE ROLE OF LORD RAM Ram | RamayanĪrun Govil plays Rama, Lord Vishnu’s seventh Dashavatara avatar in the Ramayan serial. It was also listed in the Limca Book of Records as “the most-watched mythological serial in the world” until June 2003.ĭD National re-aired the series on March 28, 2020, with one-hour episodes in the morning and one-hour episodes at night during the coronavirus shutdown of 21 days. THE RAMAYAN SERIAL CAST Ramayan Serial Castĭo you know which was the most-watched Ramayan serial in the globe from 1987 to 1988? The Ramayan serial. All of the characters in the cast, notably Arvind Trivedi’s Raavan, have a large fan base. Arun Govil plays Ram in Ramanand Sagar’s Ramayan serial, which also stars Dipika Chikhlia as Sita, Sunil Lahri as Laxman, and Dara Singh as Hanuman. The entire Ramayan cast has received a lot of love and recognition from their devoted followers. Star Bharat ran a recast of the 1987 Ramayan serial on their channel during the coronavirus pandemic.ĭuring the 2020 Coronavirus lockdown, the series was reaired, breaking multiple audience records around the world, including the record for the most-watched TV programme ever, with 77 million viewers on April 16, 2020.ĭipika Chikhlia Topiwala, who played Sita in the show, used Instagram to let her followers know about the upcoming rebroadcast. The Ramayan serial was re-telecast on Doordarshan National in March 2020, after a 33-year hiatus. According to reports, each episode of the series earned DD National Rs. ![]() The series received an 82 per cent audience rating, which is a new best for an Indian television series. The show is dependent on Tulsidas’ Ramcharitmanas and Valmiki’s Ramayan. ![]() Ramanand Sagar developed, wrote, and directed the Ramayan serial. The show first aired on DD National between 19. Ramayan is a mythical Indian Hindi-language television series based on ‘Ramayan’, the ancient Indian Sanskrit epic of the same name. RAMAYAN SERIAL- THE DD NATIONAL SHOW Ramayan Serial – The DD National Show You can still watch it on Disney+Hotstar in case you missed it, or want to rewatch it. It has made a reappearance in TV and OTT platforms in the pandemic period due to popular demand. One of the most popular ways in which Ramayan has been retold is the Ramayan serial, which was aired on DD National between 1987-1988. There have been different ways in which the it has been retold through ages. Rama’s righteous rule (Ram-raj) began after the couple’s triumphant return to Ayodhya, ushering in a golden age for all mankind. Sita entered the fire to prove her chastity, but was vindicated by the gods and returned to her husband. The allies launched an invasion of Lanka, killing Ravana and rescuing Sita. Ravana kidnapped Sita in the forest, and Rama collected an army of monkeys or the ‘vanara sena’ and bears to track her out. Rama, prince of Ayodhya, married the lovely princess Sita but was exiled for 14 years with her and his brother Laksmana due to his stepmother’s machinations. It has been re-enacted in dance dramas, village theatre, shadow-puppet theatre, and the annual Ram-lila (Rama-play). Some of India’s best writers and poets have recounted Rama’s story in poetic and dramatic renditions, as well as in narrative sculptures on the walls of temples and caves. It is one of the most important literary works of ancient India, and it has had a significant impact on art and culture on the Indian subcontinent and Southeast Asia, with variants of the story appearing in the Buddhist canon from the beginning. The Indian epic, which spans seven ‘kandas’ or ‘cantos’ (sections or divisions) and has 24,000 words, contains the teachings of ancient Hindu gurus. It is a Sanskrit epic about Prince Rama’s attempt to save his beloved wife Sita from Ravana’s grasp with the help of an army of monkeys. ![]() It dates from roughly 500 BCE to 100 BCE. Ramayan is said to have been written by the sage Valmiki. RAMAYAN | ORIGIN, BACKGROUND AND STORY Ramayan Origin ![]() If you want to use our tool, then please note that "The Tool Requires. And the tool is found working on all of them. Before making this Hack tool online available to download, we've tested this hack tool on many Devices. And the hack is totally undetectable, you're safe while using this Zenonia 5 Hack. The Zenonia Hack tool is created to add Unlimited amount of Zen points, Gold and Stat Point in the game. It is the only working Zenonia 5 Hack without root and Zenonia 5 Hack without jailbreak. 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I had a hard time with this on the PS2, and now, on the complete PS3, I think its actually gotten harder! I know what must be done : Luke, using Yoda's aid, must lower two tall mushrooms, so that Artoo can rocket from one to the other, accessing a gate that only he can open. ![]() Go to work, flirt with the ladies, go to sleep, repeat. In reality, you don’t have much control over your fate. You have the illusion of choice as the player. To improve your relationship with your stepsisters, compliment them, and stalk them when they attend various events. Usually, you’ll find people at home during the morning and afternoon, while they’re out in the world during the day. Whether delivering pizza, or working at the gym, practice makes perfect, and you’ll soon be earning a lot of money to spend at the local mall.Įvery character has a daily schedule that varies based on the day of the week. Money can be earned from jobs, which will pay more after you get promotions. 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The game is available in multiple languages, but this comes with offsets. Take advantage of them and perpetuate generational dysfunction in the family until your bloodline is wiped from the face of the Earth. Your two step-sisters are young, horny, and easy to manipulate. You’re the man of the house after your stepfather very mysteriously died. You are a socially disconnected bastard with warped morals, and no impulse control. Now your time has come! Get a job, man up, impress the ladies, and plug their ripe virgin slits with your cock! Download this game. For years you’ve wanted to plow their non-blood related asses. You are the horny step-brother of two gorgeous ladies. Available in English, German, French, Spanish, Portuguese, Dutch, and Russian languages. Man of the House, a porn game developed by Faerin. 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